test_rebase
testing bamii.htm
3_to_5_strand
        C C T A G^G
5_to_3_strand
        G^G A T C C
Entered
         Jan 1 1975 ...
Growth Temperature
         37 °
Microorganism
         Bacillus amyloliquefaciens H
Modified
         Jan 17 2000
Prototype
         BamHI
REBASE enzyme #
         185
Single-stranded cleavage
         n
Source
         ATCC 49763
descriptor
        Type II restriction enzyme
factoids
             Exhibits star activity
             Enzyme gene cloned.
             Enzyme gene sequenced.
             Crystal data present.
number_of_sites
        Adeno2
            3
        Lambda
            5
        PhiX174
            0
        SV40
            1
        pBR322
            1
title
        BamHI



testing cac81.htm
3_to_5_strand
        C G N^N C G
5_to_3_strand
        G C N^N G C
Entered
         Jun 12 1991 ...
Growth Temperature
         37 °
Microorganism
         Clostridium acetobutylicum ABKn8
Modified
         Aug 6 1999
Prototype
         Cac8I
REBASE enzyme #
         638
Source
         G. Reysett
descriptor
        Type II restriction enzyme
factoids
number_of_sites
        Adeno2
            285
        Lambda
            238
        PhiX174
            19
        SV40
            8
        pBR322
            31
title
        Cac8I



testing crei.htm
3_to_5_strand
        G A C C C A A G T T T T G C A G^C A C T C T G T C A A A C C 0
5_to_3_strand
        C T G G G T T C A A A A C G T C G T G A^G A C A G T T T G G 0
Comment
         Insertion site CGT^CGT
Entered
         Jan 1 1979 ...
Growth Temperature
         26 °
Microorganism
         Chlamydomonas reinhardtii
Modified
         Sep 9 1999
Prototype
         I-CreI
REBASE enzyme #
         2540
Source
         J.-D. Rochaix
descriptor
        Homing endonuclease
factoids
             Enzyme gene cloned.
             Enzyme gene sequenced.
             Crystal data present.
number_of_sites
        Adeno2
            0
        Lambda
            0
        PhiX174
            0
        SV40
            0
        pBR322
            0
title
        I-CreI



testing foki.htm
3_to_5_strand
        C C T A C N N N N N N N N N N N N N^
5_to_3_strand
        G G A T G N N N N N N N N N^N N N N
Comment
         CACCC sites also methylated somewhat
Entered
         Jan 1 1981 ...
Growth Temperature
         30 °
Microorganism
         Flavobacterium okeanokoites
Modified
         Aug 31 1999
Prototype
         FokI
REBASE enzyme #
         1056
Single-stranded cleavage
         n
Source
         IFO 12536
descriptor
        Type II restriction enzyme(subtype s)
factoids
             Enzyme gene cloned.
             Enzyme gene sequenced.
             Crystal data present.
number_of_sites
        Adeno2
            78
        Lambda
            150
        PhiX174
            8
        SV40
            11
        pBR322
            12
title
        FokI



testing pvuii.htm
3_to_5_strand
5_to_3_strand
          CAGCTG  
Base (Type of methylation)
         4 (N4-methylcytosine)
Entered
         Jan 1 1980 ...
Growth Temperature
         37 °
Microorganism
         Proteus vulgaris
Modified
         Jun 1 2000
Prototype
         PvuII
REBASE enzyme #
         3485
Source
         ATCC 13315
descriptor
        Type II methylase(subtype beta)
factoids
             Enzyme gene cloned.
             Enzyme gene sequenced.
             Crystal data present.
number_of_sites
        Adeno2
            24
        Lambda
            15
        PhiX174
            0
        SV40
            3
        pBR322
            1
title
        M.PvuII



testing taqi.htm
3_to_5_strand
5_to_3_strand
          TCGA  
Base (Type of methylation)
         4 (6-methyladenosine)
Entered
         Jan 1 1977 ...
Growth Temperature
         70 °
Microorganism
         Thermus aquaticus YTI
Modified
         Jun 1 2000
Prototype
         TaqI
REBASE enzyme #
         3520
Source
         J.I. Harris
descriptor
        Type II methylase(subtype gamma)
factoids
             Enzyme gene cloned.
             Enzyme gene sequenced.
             Crystal data present.
number_of_sites
        Adeno2
            50
        Lambda
            121
        PhiX174
            10
        SV40
            1
        pBR322
            7
title
        M.TaqI



